ABSTRACT
Introduction: Smoking is extremely toxic and has had a significant negative impact on society. One of the leading contributors to preventable illness and mortality has been found to be cigarette smoking. Aim: The goal of this study is to evaluate a few haematological metrics among smokers in Port Harcourt, Rivers State, and to verify the idea that smoking cigarettes either has a negative or favorable impact on these variables. Methodology: In this cross-sectional study, 100 participants between the ages of 20 and 45 were included, 50 of whom were smokers and 50 non-smokers. Venipuncture was used to obtain blood samples from the patients, which was then put into an EDTA vial for a full blood count (FBC) and other haematological analysis. The full blood count and erythrocyte sedimentation rate (ESR) were done using Haemo Auto Analyzer, Model XP-300 KOBE Japan by SYSMEX and the Westergreen method respectively. In order to analyze the data, Graph Prism Pad 6.2 and Microsoft Office Excel 2016 were both used. Using the student's independent t-test, a comparative study of mean and standard deviation values for the various parameters for test and reference ranges was conducted. Results: According to the findings, the average age of 50 smokers was 43.6200± 9.7250 years and that for the 50 non-smokers was 42.7800± 6.5440 years, which was statistically insignificant with p valve of 0.6130. Smokers’ haemoglobin level was 14.5080 ± 1.5590 (g/dL) and non-smokers was 12.1340 ± 0.70410 (g/dL). According to statistics, the level of Hb was significantly higher in smokers compared to non-smokers (P<0.0001). In comparison to non-smokers, the RBC count dramatically increased in smokers (P<0.0001) greater than in non-smokers at 5.2550 ± 0.6629 (x1012/L) and 4.6340 ± 0.5530 (x1012/L) respectively. While the total leucocyte count (TLC) in smokers is 8.0500 ± 1.8796 (x109/L), compared to 6.8580 ± 1.2454 (x109/L) in non-smokers. Statistics show that smokers have a higher total leucocyte count than non-smokers (P<0.0002). Smokers' platelet count is 255.7600±61.8351 (x109/L) while non-smokers' is 216.5800±35.5752 (x109/L).The study has statistically shown that smokers' platelet counts rose considerably in comparison to non-smokers (P<0.012). Conclusion: It may be concluded that uninterrupted smoking has an undue negative impact on haematological parameters such that increase occur in values of Hb, RBC, TLC and platelets. Significantly, these changes may increase the risk of serious health issues such as heart related defects, hardening of the arteries, Vaqu
ABSTRACT
Hepcidin is the major controller of systemic iron homeostasis and the role of the kidney in regulating hepcidin level is vital in the whole process of iron and hepcidin relationship. This study was aimed at evaluating serum Hepcidin level among Chronic Kidney Disease subjects accessing Healthcare in BMSH Port Harcourt Metropolis. The study was conducted in Port Harcourt at Braithwaite Memorial Specialist Hospital among 55 CKD subjects and 33 normal individuals making up the control group. Subjects were selected randomly and 5mls of blood was collected in plain bottle using venipuncture technique for laboratory assessment of hepcidin. Hepcidin was assayed using competitive ELISA method. T-test was used to compare the mean difference oh hepcidin between both groups. Result showed that there was a significant difference in hepcidin level between CKD and control groups; 52.00±36.00ng/ml for CKD group and 16.00±13.00ng/ml for control group, p<0.05. This study has shown that CKD has a significant impact in hepcidin level blood and consequently on iron regulation